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Image Search Results
Journal: BioMed Research International
Article Title: Human Mesenchymal Stem Cells Provide Protection against Radiation-Induced Liver Injury by Antioxidative Process, Vasculature Protection, Hepatocyte Differentiation, and Trophic Effects
doi: 10.1155/2013/151679
Figure Lengend Snippet: Human β -2-microglobulin immunostaining in mouse liver, 30 days after TBI. And percentage of mouse livers engrafted with hMSCs from 3 to 60 days after TBI. Immunostaining of hepatic cells (b), endothelium of portal vein (d) with human cells expressing beta-2-microglobulin stained in red, and respective negative controls ((a) and (c)). Isolated human cells can be seen between hepatic ((b) black arrow) and endothelial cells ((d) black arrow). (e) Negative control nonirradiated, noninjected with hMSCs (f).
Article Snippet: The
Techniques: Immunostaining, Expressing, Staining, Isolation, Negative Control
Journal: BioMed Research International
Article Title: Human Mesenchymal Stem Cells Provide Protection against Radiation-Induced Liver Injury by Antioxidative Process, Vasculature Protection, Hepatocyte Differentiation, and Trophic Effects
doi: 10.1155/2013/151679
Figure Lengend Snippet: In situ hybridization analysis and gene expression of hMSCs implanted in liver. (a) To determine whether implanted hMSCs in vivo expressed liver specific genes hMSCs before injection was compared to hMSC gene expression 15 days after injection. RT-PCR values are representative of at least 5 replicates. Results are expressed as a fold increase in gene expression in hMSCs in liver compared to hMSCs before injection. Values greater than one indicate upregulation of the target genes and values less than one indicate their downregulation. (b) Determination of cell fusion. Black arrow indicates human cells beta-2-microglobulin stained. FISH analysis showing the mice transcription centers (white arrow). (Original magnification ×80.) (d) Determination of growth factors secreted by hMSCs implanted in mice liver. The significance for all analyses was set at P < 0.01 (∗∗) and P < 0.001 (∗∗∗) and nonsignificance was noted as ns. Each group consisted of 5 animals ( n = 5).
Article Snippet: The
Techniques: In Situ Hybridization, Gene Expression, In Vivo, Injection, Reverse Transcription Polymerase Chain Reaction, Staining